Construct: shRNA TRCN0000138360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015332.2-1389s1c1
DNA Barcode:
CCCAGCAAATTGGGATCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDCD3 (23386)
Gene Description:
NudC domain containing 3
Transcript:
RefSeq NM_015332.2 (NON-CURRENT)
Match location:
Position 1389 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCAAATTGGGATCACAT
Hairpin Sequence:
5'-CCGG-CCCAGCAAATTGGGATCACAT-CTCGAG-ATGTGATCCCAATTTGCTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCAAATTGGGATCACATCTCGAGATGTGATCCCAATTTGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCAAATTGGGATCACATCTCGAGATGTGATCCCAATTTGCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000338452