Construct: shRNA TRCN0000138421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145301.1-1074s1c1
DNA Barcode:
CGAACGAACAGGGAACTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TVP23C (201158)
Gene Description:
trans-golgi network vesicle protein 23 homolog C
Transcript:
RefSeq NM_145301.1 (NON-CURRENT)
Match location:
Position 1074 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAACGAACAGGGAACTTGTA
Hairpin Sequence:
5'-CCGG-CGAACGAACAGGGAACTTGTA-CTCGAG-TACAAGTTCCCTGTTCGTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAACGAACAGGGAACTTGTACTCGAGTACAAGTTCCCTGTTCGTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAACGAACAGGGAACTTGTACTCGAGTACAAGTTCCCTGTTCGTTCG-3'

Other clones with same target sequence:

(none)