Construct: shRNA TRCN0000138502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020925.1-3631s1c1
DNA Barcode:
CCCAAGAAATGTCAGTGCGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACHD1 (57685)
Gene Description:
cache domain containing 1
Transcript:
RefSeq NM_020925.1 (NON-CURRENT)
Match location:
Position 3631 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGAAATGTCAGTGCGTA
Hairpin Sequence:
5'-CCGG-CCCAAGAAATGTCAGTGCGTA-CTCGAG-TACGCACTGACATTTCTTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGAAATGTCAGTGCGTACTCGAGTACGCACTGACATTTCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGAAATGTCAGTGCGTACTCGAGTACGCACTGACATTTCTTGGG-3'

Other clones with same target sequence:

(none)