Construct: shRNA TRCN0000138560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052880.3-2157s1c1
DNA Barcode:
CCTAGCCATAGAGCTTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3IP1 (113791)
Gene Description:
phosphoinositide-3-kinase interacting protein 1
Transcript:
RefSeq NM_052880.3 (NON-CURRENT)
Match location:
Position 2157 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGCCATAGAGCTTCCTTT
Hairpin Sequence:
5'-CCGG-CCTAGCCATAGAGCTTCCTTT-CTCGAG-AAAGGAAGCTCTATGGCTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGCCATAGAGCTTCCTTTCTCGAGAAAGGAAGCTCTATGGCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGCCATAGAGCTTCCTTTCTCGAGAAAGGAAGCTCTATGGCTAGG-3'

Other clones with same target sequence:

(none)