Construct: shRNA TRCN0000138586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002261.1-852s1c1
DNA Barcode:
CTTCCGAGTTGTGTCTGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFYVE27 (118813)
Gene Description:
zinc finger FYVE-type containing 27
Transcript:
RefSeq NM_001002261.1 (NON-CURRENT)
Match location:
Position 852 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCGAGTTGTGTCTGAGTA
Hairpin Sequence:
5'-CCGG-CTTCCGAGTTGTGTCTGAGTA-CTCGAG-TACTCAGACACAACTCGGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCGAGTTGTGTCTGAGTACTCGAGTACTCAGACACAACTCGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCGAGTTGTGTCTGAGTACTCGAGTACTCAGACACAACTCGGAAG-3'

Other clones with same target sequence:

(none)