Construct: shRNA TRCN0000138592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022757.3-7773s1c1
DNA Barcode:
CCTTCATGTAAGCAGCACGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC14 (64770)
Gene Description:
coiled-coil domain containing 14
Transcript:
RefSeq NM_022757.3 (NON-CURRENT)
Match location:
Position 7773 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCATGTAAGCAGCACGTT
Hairpin Sequence:
5'-CCGG-CCTTCATGTAAGCAGCACGTT-CTCGAG-AACGTGCTGCTTACATGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCATGTAAGCAGCACGTTCTCGAGAACGTGCTGCTTACATGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCATGTAAGCAGCACGTTCTCGAGAACGTGCTGCTTACATGAAGG-3'

Other clones with same target sequence:

(none)