Construct: shRNA TRCN0000138654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017779.3-2453s1c1
DNA Barcode:
CCCACTGAAATCAGGGCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DEPDC1 (55635)
Gene Description:
DEP domain containing 1
Transcript:
RefSeq NM_017779.3 (NON-CURRENT)
Match location:
Position 2453 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTGAAATCAGGGCATAT
Hairpin Sequence:
5'-CCGG-CCCACTGAAATCAGGGCATAT-CTCGAG-ATATGCCCTGATTTCAGTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTGAAATCAGGGCATATCTCGAGATATGCCCTGATTTCAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTGAAATCAGGGCATATCTCGAGATATGCCCTGATTTCAGTGGG-3'

Other clones with same target sequence:

(none)