Construct: shRNA TRCN0000138672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016201.2-1928s1c1
DNA Barcode:
GTCAGAACAACTGCGAGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMOTL2 (51421)
Gene Description:
angiomotin like 2
Transcript:
RefSeq NM_016201.2 (NON-CURRENT)
Match location:
Position 1928 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGAACAACTGCGAGAGAA
Hairpin Sequence:
5'-CCGG-GTCAGAACAACTGCGAGAGAA-CTCGAG-TTCTCTCGCAGTTGTTCTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGAACAACTGCGAGAGAACTCGAGTTCTCTCGCAGTTGTTCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGAACAACTGCGAGAGAACTCGAGTTCTCTCGCAGTTGTTCTGAC-3'

Other clones with same target sequence:

(none)