Construct: shRNA TRCN0000138679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175921.4-611s1c1
DNA Barcode:
GTGACATTCATCTGCTGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C5orf51 (285636)
Gene Description:
chromosome 5 open reading frame 51
Transcript:
RefSeq NM_175921.4 (NON-CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACATTCATCTGCTGGCTA
Hairpin Sequence:
5'-CCGG-GTGACATTCATCTGCTGGCTA-CTCGAG-TAGCCAGCAGATGAATGTCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACATTCATCTGCTGGCTACTCGAGTAGCCAGCAGATGAATGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACATTCATCTGCTGGCTACTCGAGTAGCCAGCAGATGAATGTCAC-3'

Other clones with same target sequence:

(none)