Construct: shRNA TRCN0000138769

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024857.3-2101s1c1
DNA Barcode:
GCTAAATGTTTCCACGCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATAD5 (79915)
Gene Description:
ATPase family AAA domain containing 5
Transcript:
RefSeq NM_024857.3 (NON-CURRENT)
Match location:
Position 2101 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138769)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAATGTTTCCACGCCCAA
Hairpin Sequence:
5'-CCGG-GCTAAATGTTTCCACGCCCAA-CTCGAG-TTGGGCGTGGAAACATTTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAATGTTTCCACGCCCAACTCGAGTTGGGCGTGGAAACATTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAATGTTTCCACGCCCAACTCGAGTTGGGCGTGGAAACATTTAGC-3'

Other clones with same target sequence:

(none)