Construct: shRNA TRCN0000138888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014744.1-3492s1c1
DNA Barcode:
GCAAAGGTGATGGACACTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D5 (9779)
Gene Description:
TBC1 domain family member 5
Transcript:
RefSeq NM_014744.1 (NON-CURRENT)
Match location:
Position 3492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGGTGATGGACACTCAT
Hairpin Sequence:
5'-CCGG-GCAAAGGTGATGGACACTCAT-CTCGAG-ATGAGTGTCCATCACCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGGTGATGGACACTCATCTCGAGATGAGTGTCCATCACCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGGTGATGGACACTCATCTCGAGATGAGTGTCCATCACCTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000281193