Construct: shRNA TRCN0000138948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145804.1-1308s1c1
DNA Barcode:
GCAAACTTGGACATCCAGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABTB2 (25841)
Gene Description:
ankyrin repeat and BTB domain containing 2
Transcript:
RefSeq NM_145804.1 (NON-CURRENT)
Match location:
Position 1308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACTTGGACATCCAGGTT
Hairpin Sequence:
5'-CCGG-GCAAACTTGGACATCCAGGTT-CTCGAG-AACCTGGATGTCCAAGTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACTTGGACATCCAGGTTCTCGAGAACCTGGATGTCCAAGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACTTGGACATCCAGGTTCTCGAGAACCTGGATGTCCAAGTTTGC-3'

Other clones with same target sequence:

(none)