Construct: shRNA TRCN0000138949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198526.1-1782s1c1
DNA Barcode:
GAAGTCCTTCAACCGCATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF710 (374655)
Gene Description:
zinc finger protein 710
Transcript:
RefSeq NM_198526.1 (NON-CURRENT)
Match location:
Position 1782 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTCCTTCAACCGCATGTA
Hairpin Sequence:
5'-CCGG-GAAGTCCTTCAACCGCATGTA-CTCGAG-TACATGCGGTTGAAGGACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTCCTTCAACCGCATGTACTCGAGTACATGCGGTTGAAGGACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTCCTTCAACCGCATGTACTCGAGTACATGCGGTTGAAGGACTTC-3'

Other clones with same target sequence:

  1. TRCN0000282020