Construct: shRNA TRCN0000138962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002824.4-418s1c1
DNA Barcode:
CCGGAAAGAGCGAAAGAAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTMS (5763)
Gene Description:
parathymosin
Transcript:
RefSeq NM_002824.4 (NON-CURRENT)
Match location:
Position 418 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAAAGAGCGAAAGAAAGA
Hairpin Sequence:
5'-CCGG-CCGGAAAGAGCGAAAGAAAGA-CTCGAG-TCTTTCTTTCGCTCTTTCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAAAGAGCGAAAGAAAGACTCGAGTCTTTCTTTCGCTCTTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAAAGAGCGAAAGAAAGACTCGAGTCTTTCTTTCGCTCTTTCCGG-3'

Other clones with same target sequence:

(none)