Construct: shRNA TRCN0000138967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014453.2-489s1c1
DNA Barcode:
CACCATGAACAGACAGCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHMP2A (27243)
Gene Description:
charged multivesicular body protein 2A
Transcript:
RefSeq NM_014453.2 (NON-CURRENT)
Match location:
Position 489 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCATGAACAGACAGCTGAA
Hairpin Sequence:
5'-CCGG-CACCATGAACAGACAGCTGAA-CTCGAG-TTCAGCTGTCTGTTCATGGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCATGAACAGACAGCTGAACTCGAGTTCAGCTGTCTGTTCATGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCATGAACAGACAGCTGAACTCGAGTTCAGCTGTCTGTTCATGGTG-3'

Other clones with same target sequence:

(none)