Construct: shRNA TRCN0000138968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014623.1-78s1c1
DNA Barcode:
CAACAGTAGTTCTAGGAGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEA1 (4201)
Gene Description:
male-enhanced antigen 1
Transcript:
RefSeq NM_014623.1 (NON-CURRENT)
Match location:
Position 78 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGTAGTTCTAGGAGGAG
Hairpin Sequence:
5'-CCGG-CAACAGTAGTTCTAGGAGGAG-CTCGAG-CTCCTCCTAGAACTACTGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGTAGTTCTAGGAGGAGCTCGAGCTCCTCCTAGAACTACTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGTAGTTCTAGGAGGAGCTCGAGCTCCTCCTAGAACTACTGTTG-3'

Other clones with same target sequence:

(none)