Construct: shRNA TRCN0000138988

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153233.1-2142s1c1
DNA Barcode:
CCTTATCCTCTTCCTCCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNE4 (163183)
Gene Description:
spectrin repeat containing nuclear envelope family member 4
Transcript:
RefSeq NM_153233.1 (NON-CURRENT)
Match location:
Position 2142 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138988)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTATCCTCTTCCTCCTCTT
Hairpin Sequence:
5'-CCGG-CCTTATCCTCTTCCTCCTCTT-CTCGAG-AAGAGGAGGAAGAGGATAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTATCCTCTTCCTCCTCTTCTCGAGAAGAGGAGGAAGAGGATAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTATCCTCTTCCTCCTCTTCTCGAGAAGAGGAGGAAGAGGATAAGG-3'

Other clones with same target sequence:

(none)