Construct: shRNA TRCN0000139010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130388.2-479s1c1
DNA Barcode:
CTAAACTACCAGTCTGGGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASB12 (142689)
Gene Description:
ankyrin repeat and SOCS box containing 12
Transcript:
RefSeq NM_130388.2 (NON-CURRENT)
Match location:
Position 479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAACTACCAGTCTGGGCAT
Hairpin Sequence:
5'-CCGG-CTAAACTACCAGTCTGGGCAT-CTCGAG-ATGCCCAGACTGGTAGTTTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAACTACCAGTCTGGGCATCTCGAGATGCCCAGACTGGTAGTTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAACTACCAGTCTGGGCATCTCGAGATGCCCAGACTGGTAGTTTAG-3'

Other clones with same target sequence:

(none)