Construct: shRNA TRCN0000139073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017864.1-1037s1c1
DNA Barcode:
CACAATGTTCGGGAGGACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INTS8 (55656)
Gene Description:
integrator complex subunit 8
Transcript:
RefSeq NM_017864.1 (NON-CURRENT)
Match location:
Position 1037 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAATGTTCGGGAGGACATT
Hairpin Sequence:
5'-CCGG-CACAATGTTCGGGAGGACATT-CTCGAG-AATGTCCTCCCGAACATTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAATGTTCGGGAGGACATTCTCGAGAATGTCCTCCCGAACATTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAATGTTCGGGAGGACATTCTCGAGAATGTCCTCCCGAACATTGTG-3'

Other clones with same target sequence:

(none)