Construct: shRNA TRCN0000139143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015172.2-10209s1c1
DNA Barcode:
CCCGGTTTACTGTGTTGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRRC2C (23215)
Gene Description:
proline rich coiled-coil 2C
Transcript:
RefSeq NM_015172.2 (NON-CURRENT)
Match location:
Position 10209 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGTTTACTGTGTTGAGTT
Hairpin Sequence:
5'-CCGG-CCCGGTTTACTGTGTTGAGTT-CTCGAG-AACTCAACACAGTAAACCGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGTTTACTGTGTTGAGTTCTCGAGAACTCAACACAGTAAACCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGTTTACTGTGTTGAGTTCTCGAGAACTCAACACAGTAAACCGGG-3'

Other clones with same target sequence:

(none)