Construct: shRNA TRCN0000139146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016353.2-1823s1c1
DNA Barcode:
CCTCAGACTGAGTGAACTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZDHHC2 (51201)
Gene Description:
zinc finger DHHC-type palmitoyltransferase 2
Transcript:
RefSeq NM_016353.2 (NON-CURRENT)
Match location:
Position 1823 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGACTGAGTGAACTGTA
Hairpin Sequence:
5'-CCGG-CCTCAGACTGAGTGAACTGTA-CTCGAG-TACAGTTCACTCAGTCTGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGACTGAGTGAACTGTACTCGAGTACAGTTCACTCAGTCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGACTGAGTGAACTGTACTCGAGTACAGTTCACTCAGTCTGAGG-3'

Other clones with same target sequence:

(none)