Construct: shRNA TRCN0000139414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019607.1-1098s1c1
DNA Barcode:
CCACTGACACACAAGCACTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C8orf44 (56260)
Gene Description:
chromosome 8 open reading frame 44
Transcript:
RefSeq NM_019607.1 (NON-CURRENT)
Match location:
Position 1098 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGACACACAAGCACTAA
Hairpin Sequence:
5'-CCGG-CCACTGACACACAAGCACTAA-CTCGAG-TTAGTGCTTGTGTGTCAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGACACACAAGCACTAACTCGAGTTAGTGCTTGTGTGTCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGACACACAAGCACTAACTCGAGTTAGTGCTTGTGTGTCAGTGG-3'

Other clones with same target sequence:

(none)