Construct: shRNA TRCN0000139447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006816.1-131s1c1
DNA Barcode:
CGGATATAACTGACGGCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMAN2 (10960)
Gene Description:
lectin, mannose binding 2
Transcript:
RefSeq NM_006816.1 (NON-CURRENT)
Match location:
Position 131 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATATAACTGACGGCAACA
Hairpin Sequence:
5'-CCGG-CGGATATAACTGACGGCAACA-CTCGAG-TGTTGCCGTCAGTTATATCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATATAACTGACGGCAACACTCGAGTGTTGCCGTCAGTTATATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATATAACTGACGGCAACACTCGAGTGTTGCCGTCAGTTATATCCG-3'

Other clones with same target sequence:

(none)