Construct: shRNA TRCN0000139479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152613.1-991s1c1
DNA Barcode:
CCAAGCAAAGAGGTACCCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WBP2NL (164684)
Gene Description:
WBP2 N-terminal like
Transcript:
RefSeq NM_152613.1 (NON-CURRENT)
Match location:
Position 991 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCAAAGAGGTACCCTAA
Hairpin Sequence:
5'-CCGG-CCAAGCAAAGAGGTACCCTAA-CTCGAG-TTAGGGTACCTCTTTGCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCAAAGAGGTACCCTAACTCGAGTTAGGGTACCTCTTTGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCAAAGAGGTACCCTAACTCGAGTTAGGGTACCTCTTTGCTTGG-3'

Other clones with same target sequence:

(none)