Construct: shRNA TRCN0000139520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017927.1-561s1c1
DNA Barcode:
CCGTTCCTTTGAACATCGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C2orf76 (130355)
Gene Description:
chromosome 2 open reading frame 76
Transcript:
RefSeq NM_001017927.1 (NON-CURRENT)
Match location:
Position 561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTCCTTTGAACATCGCAA
Hairpin Sequence:
5'-CCGG-CCGTTCCTTTGAACATCGCAA-CTCGAG-TTGCGATGTTCAAAGGAACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTCCTTTGAACATCGCAACTCGAGTTGCGATGTTCAAAGGAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTCCTTTGAACATCGCAACTCGAGTTGCGATGTTCAAAGGAACGG-3'

Other clones with same target sequence:

(none)