Construct: shRNA TRCN0000139528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015433.2-662s1c1
DNA Barcode:
CTCCAAGATGAGAAAGGAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF1AKMT3 (25895)
Gene Description:
EEF1A lysine methyltransferase 3
Transcript:
RefSeq NM_015433.2 (NON-CURRENT)
Match location:
Position 662 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAAGATGAGAAAGGAGCA
Hairpin Sequence:
5'-CCGG-CTCCAAGATGAGAAAGGAGCA-CTCGAG-TGCTCCTTTCTCATCTTGGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAAGATGAGAAAGGAGCACTCGAGTGCTCCTTTCTCATCTTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAAGATGAGAAAGGAGCACTCGAGTGCTCCTTTCTCATCTTGGAG-3'

Other clones with same target sequence:

(none)