Construct: shRNA TRCN0000139529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018712.2-462s1c1
DNA Barcode:
CCTTCTGCAAATCGTTGGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELMOD1 (55531)
Gene Description:
ELMO domain containing 1
Transcript:
RefSeq NM_018712.2 (NON-CURRENT)
Match location:
Position 462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTGCAAATCGTTGGGTA
Hairpin Sequence:
5'-CCGG-CCTTCTGCAAATCGTTGGGTA-CTCGAG-TACCCAACGATTTGCAGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTGCAAATCGTTGGGTACTCGAGTACCCAACGATTTGCAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTGCAAATCGTTGGGTACTCGAGTACCCAACGATTTGCAGAAGG-3'

Other clones with same target sequence:

(none)