Construct: shRNA TRCN0000139603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015075.1-2531s1c1
DNA Barcode:
CGTACAGTTTCCGTCAGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQSEC2 (23096)
Gene Description:
IQ motif and Sec7 domain ArfGEF 2
Transcript:
RefSeq NM_015075.1 (NON-CURRENT)
Match location:
Position 2531 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACAGTTTCCGTCAGTCTT
Hairpin Sequence:
5'-CCGG-CGTACAGTTTCCGTCAGTCTT-CTCGAG-AAGACTGACGGAAACTGTACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACAGTTTCCGTCAGTCTTCTCGAGAAGACTGACGGAAACTGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACAGTTTCCGTCAGTCTTCTCGAGAAGACTGACGGAAACTGTACG-3'

Other clones with same target sequence:

(none)