Construct: shRNA TRCN0000139661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145315.2-1576s1c1
DNA Barcode:
CTCACGGAAATGCAGACTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AFG1L (246269)
Gene Description:
AFG1 like ATPase
Transcript:
RefSeq NM_145315.2 (NON-CURRENT)
Match location:
Position 1576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACGGAAATGCAGACTGAA
Hairpin Sequence:
5'-CCGG-CTCACGGAAATGCAGACTGAA-CTCGAG-TTCAGTCTGCATTTCCGTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACGGAAATGCAGACTGAACTCGAGTTCAGTCTGCATTTCCGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACGGAAATGCAGACTGAACTCGAGTTCAGTCTGCATTTCCGTGAG-3'

Other clones with same target sequence:

(none)