Construct: shRNA TRCN0000139672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003504.3-614s1c1
DNA Barcode:
CGGAGAAGAGACATCCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC45 (8318)
Gene Description:
cell division cycle 45
Transcript:
RefSeq NM_003504.3 (NON-CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAAGAGACATCCTCTTT
Hairpin Sequence:
5'-CCGG-CGGAGAAGAGACATCCTCTTT-CTCGAG-AAAGAGGATGTCTCTTCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAAGAGACATCCTCTTTCTCGAGAAAGAGGATGTCTCTTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAAGAGACATCCTCTTTCTCGAGAAAGAGGATGTCTCTTCTCCG-3'

Other clones with same target sequence:

(none)