Construct: shRNA TRCN0000139730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004699.1-774s1c1
DNA Barcode:
CCTTCGGAAAGACTTCAGTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM50A (9130)
Gene Description:
family with sequence similarity 50 member A
Transcript:
RefSeq NM_004699.1 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGGAAAGACTTCAGTGA
Hairpin Sequence:
5'-CCGG-CCTTCGGAAAGACTTCAGTGA-CTCGAG-TCACTGAAGTCTTTCCGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGGAAAGACTTCAGTGACTCGAGTCACTGAAGTCTTTCCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGGAAAGACTTCAGTGACTCGAGTCACTGAAGTCTTTCCGAAGG-3'

Other clones with same target sequence:

(none)