Construct: shRNA TRCN0000139752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002107.2-166s1c1
DNA Barcode:
CAGGAAGCAACTGGCTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3-3A (3020)
Gene Description:
H3.3 histone A
Transcript:
RefSeq NM_002107.2 (NON-CURRENT)
Match location:
Position 166 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAGCAACTGGCTACAAA
Hairpin Sequence:
5'-CCGG-CAGGAAGCAACTGGCTACAAA-CTCGAG-TTTGTAGCCAGTTGCTTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAGCAACTGGCTACAAACTCGAGTTTGTAGCCAGTTGCTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAGCAACTGGCTACAAACTCGAGTTTGTAGCCAGTTGCTTCCTG-3'

Other clones with same target sequence:

(none)