Construct: shRNA TRCN0000139825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032866.2-3454s1c1
DNA Barcode:
CAAGGACTTAAAGAGCCGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CGNL1 (84952)
Gene Description:
cingulin like 1
Transcript:
RefSeq NM_032866.2 (NON-CURRENT)
Match location:
Position 3454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGACTTAAAGAGCCGGAT
Hairpin Sequence:
5'-CCGG-CAAGGACTTAAAGAGCCGGAT-CTCGAG-ATCCGGCTCTTTAAGTCCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGACTTAAAGAGCCGGATCTCGAGATCCGGCTCTTTAAGTCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGACTTAAAGAGCCGGATCTCGAGATCCGGCTCTTTAAGTCCTTG-3'

Other clones with same target sequence:

(none)