Construct: shRNA TRCN0000139861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203426.1-1422s1c1
DNA Barcode:
CTCTGGAATCCAGTCCCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLGA2P7 (388152)
Gene Description:
GOLGA2 pseudogene 7
Transcript:
RefSeq NM_203426.1 (NON-CURRENT)
Match location:
Position 1422 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGGAATCCAGTCCCATTT
Hairpin Sequence:
5'-CCGG-CTCTGGAATCCAGTCCCATTT-CTCGAG-AAATGGGACTGGATTCCAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGGAATCCAGTCCCATTTCTCGAGAAATGGGACTGGATTCCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGGAATCCAGTCCCATTTCTCGAGAAATGGGACTGGATTCCAGAG-3'

Other clones with same target sequence:

(none)