Construct: shRNA TRCN0000139867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014814.1-1016s1c1
DNA Barcode:
GATCAGGAACTGTCCAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMD6 (9861)
Gene Description:
proteasome 26S subunit, non-ATPase 6
Transcript:
RefSeq NM_014814.1 (NON-CURRENT)
Match location:
Position 1016 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGGAACTGTCCAGGTTT
Hairpin Sequence:
5'-CCGG-GATCAGGAACTGTCCAGGTTT-CTCGAG-AAACCTGGACAGTTCCTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGGAACTGTCCAGGTTTCTCGAGAAACCTGGACAGTTCCTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGGAACTGTCCAGGTTTCTCGAGAAACCTGGACAGTTCCTGATC-3'

Other clones with same target sequence:

(none)