Construct: shRNA TRCN0000139879

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012979.1-479s1c1
DNA Barcode:
GATGAGGGACAACTGGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCEAL5 (340543)
Gene Description:
transcription elongation factor A like 5
Transcript:
RefSeq NM_001012979.1 (NON-CURRENT)
Match location:
Position 479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139879)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGGGACAACTGGAAGAT
Hairpin Sequence:
5'-CCGG-GATGAGGGACAACTGGAAGAT-CTCGAG-ATCTTCCAGTTGTCCCTCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGGGACAACTGGAAGATCTCGAGATCTTCCAGTTGTCCCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGGGACAACTGGAAGATCTCGAGATCTTCCAGTTGTCCCTCATC-3'

Other clones with same target sequence:

  1. TRCN0000137398