Construct: shRNA TRCN0000139923

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015411.1-218s1c1
DNA Barcode:
GACAGTGAAACCCTTTGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUMF2 (25870)
Gene Description:
sulfatase modifying factor 2
Transcript:
RefSeq NM_015411.1 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139923)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGTGAAACCCTTTGCCAT
Hairpin Sequence:
5'-CCGG-GACAGTGAAACCCTTTGCCAT-CTCGAG-ATGGCAAAGGGTTTCACTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGTGAAACCCTTTGCCATCTCGAGATGGCAAAGGGTTTCACTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGTGAAACCCTTTGCCATCTCGAGATGGCAAAGGGTTTCACTGTC-3'

Other clones with same target sequence:

(none)