Construct: shRNA TRCN0000139941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018031.2-3859s1c1
DNA Barcode:
GCTTCCTTTGCCTTTACCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR6 (11180)
Gene Description:
WD repeat domain 6
Transcript:
RefSeq NM_018031.2 (NON-CURRENT)
Match location:
Position 3859 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000139941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTTTGCCTTTACCCTA
Hairpin Sequence:
5'-CCGG-GCTTCCTTTGCCTTTACCCTA-CTCGAG-TAGGGTAAAGGCAAAGGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTTTGCCTTTACCCTACTCGAGTAGGGTAAAGGCAAAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTTTGCCTTTACCCTACTCGAGTAGGGTAAAGGCAAAGGAAGC-3'

Other clones with same target sequence:

(none)