Construct: shRNA TRCN0000140052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013275.3-9044s1c1
DNA Barcode:
CCGCGTGGTTTGTATCTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD11 (29123)
Gene Description:
ankyrin repeat domain 11
Transcript:
RefSeq NM_013275.3 (NON-CURRENT)
Match location:
Position 9044 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCGTGGTTTGTATCTTCTT
Hairpin Sequence:
5'-CCGG-CCGCGTGGTTTGTATCTTCTT-CTCGAG-AAGAAGATACAAACCACGCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCGTGGTTTGTATCTTCTTCTCGAGAAGAAGATACAAACCACGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCGTGGTTTGTATCTTCTTCTCGAGAAGAAGATACAAACCACGCGG-3'

Other clones with same target sequence:

  1. TRCN0000344174