Construct: shRNA TRCN0000140070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001018059.1-925s1c1
DNA Barcode:
GAGGTGGAACAATCACCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPIPB15 (440348)
Gene Description:
nuclear pore complex interacting protein family member B15
Transcript:
RefSeq NM_001018059.1 (NON-CURRENT)
Match location:
Position 925 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGTGGAACAATCACCGAAA
Hairpin Sequence:
5'-CCGG-GAGGTGGAACAATCACCGAAA-CTCGAG-TTTCGGTGATTGTTCCACCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGTGGAACAATCACCGAAACTCGAGTTTCGGTGATTGTTCCACCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGTGGAACAATCACCGAAACTCGAGTTTCGGTGATTGTTCCACCTC-3'

Other clones with same target sequence:

(none)