Construct: shRNA TRCN0000140114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032343.1-199s1c1
DNA Barcode:
GCCTTCAAGATGGCAACTTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHCHD6 (84303)
Gene Description:
coiled-coil-helix-coiled-coil-helix domain containing 6
Transcript:
RefSeq NM_032343.1 (NON-CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCAAGATGGCAACTTGA
Hairpin Sequence:
5'-CCGG-GCCTTCAAGATGGCAACTTGA-CTCGAG-TCAAGTTGCCATCTTGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCAAGATGGCAACTTGACTCGAGTCAAGTTGCCATCTTGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCAAGATGGCAACTTGACTCGAGTCAAGTTGCCATCTTGAAGGC-3'

Other clones with same target sequence:

(none)