Construct: shRNA TRCN0000140136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017905.3-915s1c1
DNA Barcode:
GATGTAAGAAAGGCAGCGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMCO3 (55002)
Gene Description:
transmembrane and coiled-coil domains 3
Transcript:
RefSeq NM_017905.3 (NON-CURRENT)
Match location:
Position 915 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTAAGAAAGGCAGCGGAT
Hairpin Sequence:
5'-CCGG-GATGTAAGAAAGGCAGCGGAT-CTCGAG-ATCCGCTGCCTTTCTTACATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTAAGAAAGGCAGCGGATCTCGAGATCCGCTGCCTTTCTTACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTAAGAAAGGCAGCGGATCTCGAGATCCGCTGCCTTTCTTACATC-3'

Other clones with same target sequence:

(none)