Construct: shRNA TRCN0000140223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003829.1-6183s1c1
DNA Barcode:
GCCAGAATTGAACCAACCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPDZ (8777)
Gene Description:
multiple PDZ domain crumbs cell polarity complex component
Transcript:
RefSeq NM_003829.1 (NON-CURRENT)
Match location:
Position 6183 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAATTGAACCAACCCAA
Hairpin Sequence:
5'-CCGG-GCCAGAATTGAACCAACCCAA-CTCGAG-TTGGGTTGGTTCAATTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAATTGAACCAACCCAACTCGAGTTGGGTTGGTTCAATTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAATTGAACCAACCCAACTCGAGTTGGGTTGGTTCAATTCTGGC-3'

Other clones with same target sequence:

(none)