Construct: shRNA TRCN0000140243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032511.1-1809s1c1
DNA Barcode:
GCTGGTTGTCAGCTGATAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAXC (84553)
Gene Description:
failed axon connections homolog, metaxin like GST domain containing
Transcript:
RefSeq NM_032511.1 (NON-CURRENT)
Match location:
Position 1809 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTGTCAGCTGATAGTT
Hairpin Sequence:
5'-CCGG-GCTGGTTGTCAGCTGATAGTT-CTCGAG-AACTATCAGCTGACAACCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTGTCAGCTGATAGTTCTCGAGAACTATCAGCTGACAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTGTCAGCTGATAGTTCTCGAGAACTATCAGCTGACAACCAGC-3'

Other clones with same target sequence:

(none)