Construct: shRNA TRCN0000140248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207439.2-3775s1c1
DNA Barcode:
GCTCTCCAGTATGCTGTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD20A19P (400110)
Gene Description:
ankyrin repeat domain 20 family member A19, pseudogene
Transcript:
RefSeq NM_207439.2 (NON-CURRENT)
Match location:
Position 3775 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCCAGTATGCTGTGTAT
Hairpin Sequence:
5'-CCGG-GCTCTCCAGTATGCTGTGTAT-CTCGAG-ATACACAGCATACTGGAGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCCAGTATGCTGTGTATCTCGAGATACACAGCATACTGGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCCAGTATGCTGTGTATCTCGAGATACACAGCATACTGGAGAGC-3'

Other clones with same target sequence:

(none)