Construct: shRNA TRCN0000140270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022464.3-308s1c1
DNA Barcode:
GTACGGCTGAATCTTCAGACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIL1 (64374)
Gene Description:
SIL1 nucleotide exchange factor
Transcript:
RefSeq NM_022464.3 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGGCTGAATCTTCAGACT
Hairpin Sequence:
5'-CCGG-GTACGGCTGAATCTTCAGACT-CTCGAG-AGTCTGAAGATTCAGCCGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGGCTGAATCTTCAGACTCTCGAGAGTCTGAAGATTCAGCCGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGGCTGAATCTTCAGACTCTCGAGAGTCTGAAGATTCAGCCGTAC-3'

Other clones with same target sequence:

(none)