Construct: shRNA TRCN0000140338
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032866.2-491s1c1
- DNA Barcode:
- CCATCTCACCTGCTGAACTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CGNL1 (84952)
- Gene Description:
- cingulin like 1
- Transcript:
- RefSeq NM_032866.2 (NON-CURRENT)
- Match location:
- Position 491 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000140338)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATCTCACCTGCTGAACTTT
- Hairpin Sequence:
-
5'-CCGG-CCATCTCACCTGCTGAACTTT-CTCGAG-AAAGTTCAGCAGGTGAGATGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATCTCACCTGCTGAACTTTCTCGAGAAAGTTCAGCAGGTGAGATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATCTCACCTGCTGAACTTTCTCGAGAAAGTTCAGCAGGTGAGATGG-3'
Other clones with same target sequence:
(none)
