Construct: shRNA TRCN0000140442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014851.2-1516s1c1
DNA Barcode:
GAGGAACGAATGGGACAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLHL21 (9903)
Gene Description:
kelch like family member 21
Transcript:
RefSeq NM_014851.2 (NON-CURRENT)
Match location:
Position 1516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAACGAATGGGACAAGAT
Hairpin Sequence:
5'-CCGG-GAGGAACGAATGGGACAAGAT-CTCGAG-ATCTTGTCCCATTCGTTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAACGAATGGGACAAGATCTCGAGATCTTGTCCCATTCGTTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAACGAATGGGACAAGATCTCGAGATCTTGTCCCATTCGTTCCTC-3'

Other clones with same target sequence:

(none)