Construct: shRNA TRCN0000140520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001383.1-810s1c1
DNA Barcode:
GCTTACCGGTATGACCCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPH1 (1801)
Gene Description:
diphthamide biosynthesis 1
Transcript:
RefSeq NM_001383.1 (NON-CURRENT)
Match location:
Position 810 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTACCGGTATGACCCATAT
Hairpin Sequence:
5'-CCGG-GCTTACCGGTATGACCCATAT-CTCGAG-ATATGGGTCATACCGGTAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTACCGGTATGACCCATATCTCGAGATATGGGTCATACCGGTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTACCGGTATGACCCATATCTCGAGATATGGGTCATACCGGTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000292309