Construct: shRNA TRCN0000140585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199000.1-245s1c1
DNA Barcode:
GCCATCTTCACCATCTGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LHFPL3 (375612)
Gene Description:
LHFPL tetraspan subfamily member 3
Transcript:
RefSeq NM_199000.1 (NON-CURRENT)
Match location:
Position 245 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCTTCACCATCTGCTTT
Hairpin Sequence:
5'-CCGG-GCCATCTTCACCATCTGCTTT-CTCGAG-AAAGCAGATGGTGAAGATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCTTCACCATCTGCTTTCTCGAGAAAGCAGATGGTGAAGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCTTCACCATCTGCTTTCTCGAGAAAGCAGATGGTGAAGATGGC-3'

Other clones with same target sequence:

(none)